ChapterDx Microbial Identification NGS Assay
ChapterDx Microbial ID NGS assay is a single-tube and single-PCR reaction and has a broad bacterial identification range. The assay is highly sensitive and specific, and detects low-abundance bacteria as well as pathogenic bacteria. It is suitable for microbiome identification to analyze the diversity and composition of microbial communities as well as disease association.
Description
ChapterDx Microbial ID NGS assay is a highly multiplex PCR assay that amplifies and analyzes bacterial 16S gene. The assay is a single-tube and single-step PCR reaction, where amplification and indexing take places simultaneously in the same reaction. The assay can be used for bacterial detection and microbiome studies to analyze the diversity and composition of microbial communities in various environments, including the human gut, soil, water, and more. Moreover, this assay can be applied for bacterial pathogen identification as well as disease association in understanding the role of microbial communities in health and disease. The assay comes with data analysis software.
Technology
ChapterDx Microbial ID NGS assay consists of the following steps:
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DNA extraction (not provided)
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Multiplex PCR
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Pooling and purification
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Next-generation sequencing
In the PCR reaction, both amplification and indexing/barcoding of each specimen occurs simultaneously. After PCR reaction, amplicons for different samples are pooled in one tube. The following procedures deal with only one tube regardless of sample numbers. After the cleanup step, the library is sequenced on an Illumina sequencing platform. The entire workflow takes about 24 hours, of which about 3 hours is benchwork/incubation time and 17 hours of sequencing. It takes about half hour to complete data analysis.
Features
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Designed to target 16S regions with selective detection of pathogenic bacteria
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Broad identification range
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Wide range of applications such as environmental microbiome, human microbiome, pathogen identification, food contamination, soil microbiome, and etc
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Single-tube and single-step PCR
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User-friendly workflow and easy to automate
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Cost-efficient, and suitable for low and high-throughput sample scales
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Data analysis software included
ChapterDx Microbial ID NGS Assay Workflow
