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ChapterDx Dual Bacteria and Fungi Identification NGS Assay 

ChapterDx Dual Bacteria and Fungi Identification NGS assay is a single-tube and single-PCR reaction. The assay detects and identifies a broad range of bacterial and fungal species and ha s wide range of applications.

Description

ChapterDx Dual Bacteria and Fungi ID NGS assay is a highly multiplex PCR assay that amplifies and analyzes 16S and ITS1/2 regionsThe assay is a single-tube and single-step PCR reaction, where amplification and indexing take places simultaneously in the same reaction. The assay can be used for bacteria and fungi detection in a wide range of applications. One application is food and beverage industries such as wine microbiome. Combined 16S and ITS sequencing is a powerful tool for bacterial and fungal identification and biodiversity studies, offering broad applicabilit and valuable insights.The assay comes with data analysis software.

Technology

ChapterDx Dual Bacteria and Fungi ID NGS assay  consists of the following steps:

  1. DNA extraction (not provided)

  2. Multiplex PCR

  3. Pooling and purification

  4. Next-generation sequencing

In the PCR reaction, both amplification and indexing/barcoding of each specimen occurs simultaneously. After PCR reaction, amplicons for different samples are pooled in one tube. The following procedures deal with only one tube regardless of sample numbers. After the cleanup step, the library is sequenced on an Illumina sequencing platform. The entire workflow takes about 24 hours, of which about 3 hours is benchwork/incubation time and 17 hours of sequencing. It takes about half hour to complete data analysis.

Features

  • Designed to target 16S and ITS regions

  • Broad identification range of bacteria and fungi

  • Wide range of applications such as environmental microbiome, human microbiome, pathogen identification, food and beverage such as wine microbiome, beta soil microbiome, and etc

  • Single-tube and single-step PCR

  • User-friendly workflow and easy to automate

  • Cost-efficient, and suitable for low and high-throughput sample scales

  • Data analysis software included

ChapterDx Dual Bacteria and Fungi ID NGS Assay Workflow

Salmonella Serotyping workflow.png

Cat No./ Size     CBF7853A /96 rxns
                          (includes12 indexing per sequencing run*)                                                 
                           *More indexing options are available
   

For Research Use Only (RUO)

Patent Pending

1455 Adams Dr #2162

Menlo Park, CA 94025

© 2022 Chapter Diagnostics Inc.  All rights reserved.

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