ChapterDX MLSTnext™ Cyclospora Target Enrichment
Reliable identification in just 14 hours
<14 Hours
The total workflow is designed for efficiency, requiring less than 14 hours to complete. This timeline includes 6 hours of benchwork and incubation time, approximately 7 hours of sequencing, and less than 30 minutes for automated data analysis.
Low amount of cDNA
This assay requires low amounts of input DNA and is suitable for small, medium and high-throughput sample scales.
Technology
The Chapter Diagnostics MLSTnext™ Cyclospora target enrichment NGS kit offers a high-resolution, multiplexed solution for identifying Cyclospora variants. By targeting specific informative loci across the genome, this kit replaces traditional, labor-intensive subtyping with a streamlined Next-Generation Sequencing (NGS) approach optimized for Illumina platforms.
Optimized Workflow
The assay is designed for maximum efficiency, following a logical seven-step progression:
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DNA extraction (not provided)
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First PCR
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Pooling and purification
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Target enrichment
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Second PCR
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Pooling and purification
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Next-generation sequencing
With a total turnaround time of less than 14 hours—comprising 6 hours of laboratory procedures, 7 hours of sequencing, and 30 minutes of data analysis—this assay is designed for high sensitivity with low DNA input. Its flexible architecture is equally suited for small, medium, and high-throughput sample scales, offering a rapid and scalable solution for modern molecular diagnostics.
ChapterDx MLSTnext™ Cyclospora Target Enrichment NGS Assay Workflow
