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ChapterDx MLSTnext Cyclospora Genotyping NGS Assay 

ChapterDx MLSTnext Cyclospora Genotyping NGS assay analyzes over 50 polymorphic loci spanning the entire Cyclospora genome. The combination of these highly polymorphic target loci enables high-resolution typing of Cyclospora cayetanensis, enable tracking-and-tracing source of contamination. For low copy number Cyclospora specimens, additional target enrichment reagents are available to boost the detection sensitivity.
 

Description

ChapterDx MLSTnext Cyclospora genotyping NGS assay is a unique and highly multiplex PCR assay that amplifies and analyzes over 50 polymorphic loci spanning Cyclospora genome. The assay is a single-tube and single-step PCR reaction, where amplification and indexing take place simultaneously in the same reaction.  This assay is able to track-and-trace source of contamination. Additionally, ChapterDX offers target enrichment reagents for low copy number specimen. The Cyclospora target enrichment steps substantially increase the detection sensitivity. The data analysis software is included.

Technology

ChapterDx Cyclospora genotyping NGS Assay consists of the following steps:

  1. DNA extraction (not provided)

  2. Multiplex PCR

  3. Pooling and purification

  4. Next-generation sequencing

In the PCR reaction, both amplification and indexing/barcoding of each specimen occurs simultaneously. After PCR reaction, amplicons for different samples are pooled in one tube. The following procedures deal with only one tube regardless of sample number. After the cleanup step, the library is sequenced on an Illumina sequencing platform. The entire workflow takes about 24 hours, of which about 3 hours is benchwork/incubation time and 17 hours of sequencing. It takes less than half hour to complete data analysis.

Features

  • Sequence analysis of over 50 polymorphic loci across Cyclospora genome

  • Provides high-resolution typing results

  • Excellent tool for tracking-and-tracing the source of contamination

  • Single-tube and single-step PCR

  • User-friendly workflow and easy to automate

  • Cost-efficient, and suitable for low to high-throughput scales

  • Data analysis software included

  • Target enrichment solutions for low copy number specimens 

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ChapterDx Cyclospora Genotyping NGS Assay Workflow

Salmonella Serotyping workflow.png

Cat No./ Size     C7229A /96 rxns (including 96 indexing)                                                
                          C2729B /384 rxns (including 384 indexing)

For Research Use Only (RUO)

Patent Pending

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