ChapterDx Comprehensive HPV-STI NGS Kit
Detection, identification and quantification of 41 HPV-STI types/species (28 HPV and 13 STI) including internal control in a single-tube and single-PCR reaction. Simple and user-friendly workflow, extremely low-cost and easy to automate
Description
ChapterDx comprehensive HPV-STI assay is a proprietary NGS-based method that detects, genotypes and quantifies 28 HPVs and 13 STIs in a specimen. It is a single-tube and single-PCR method using type/species-specific primers for uniform amplification. Indexing/barcoding and amplification of each sample occur simultaneously in the PCR reaction. It requires low amounts of DNA, has relatively fast turnaround time and is suitable for medium- and high-throughput sample scales (96 to 10,000 samples per sequencing run). The assay is extremely low-cost, has a simple and user-friendly workflow and is easy to automate. The kit includes a data analysis software.
Technology
ChapterDx HPV -STI assay consists of the following steps: i) DNA extraction; ii) highly multiplex PCR; iii) pooling and purification; and iv) next-generation sequencing. In the PCR reaction, both amplification and indexing/barcoding of each specimen occur simultaneously. After the PCR, all the amplicons are pooled in one tube. From this point, the downstream procedure deals with only one tube regardless of the sample number. The pooled amplicons are purified in a cleanup step and thereafter, the library is sequenced on an Illumina sequencing platform. The sample capacity for a MiniSeq Mid Output kit is minimum 400 samples. The entire workflow takes about 24 hours of which about 6 hours is benchwork/incubation time and 17 hours of sequencing. Data analysis takes 10 minutes. The developed dual-indexing can be used for up to 10,000 samples per sequencing run.
Features
- Detection and identification of 41 STI (28 HPVs and 13 STIs) and Chlamydia serovars L1, L2, B, D, E, F & G
- Single-tube and single PCR reaction test
- Quantitative (viral load/copy number)
- Type/species-specific amplification
- User-friendly workflow and easy to automate
- Suitable for medium and high throughput scales
- High sensitivity and specificity
- Low-cost
41 HPV & STI types/species
High-Risk HPV-16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 (68a and 68b), 26, 53, 73, 82
Low-Risk HPV-6, 11, 40, 42, 43, 44, 55, 61, 81, 83
Chlamydia trachomatis (CT)
Neisseria gonorrhoeae (NG)
Mycoplasma genitalium (MG)
Trichomonas vaginalis (TV)
Treponema pallidum (TP)
Herpes simplex virus type 1 (HSV-1)
Herpes simplex virus type 2 (HSV-2)
Varicella zoster virus (VZV)
Haemophilus ducreyi (HD)
Chlamydia serovars: L1, L2, B, D, E, F & G
Mycoplasma hominis (MH)
Ureaplasma urealyticum (UU)
Ureaplasma parvum (UP)
ChapterDx HPV-STI Technology and Workflow Illustration
