ChapterDx MLSTnext Salmonella Serotyping NGS Assay
ChapterDx MLSTnext Salmonella serotyping NGS assay analyzes 46 most polymorphic loci spanning the entire Salmonella genome. The combination of sequences at those loci enables not only distinguishing most salmonella serotypes, but also discriminating species of the same serotype. In addition, this assay is able to detect up to four salmonella species of different serotypes from same sample.
Description
ChapterDx MLSTnext Salmonella serotyping NGS assay is a unique and highly multiplex PCR assay that amplifies and analyzes 46 most polymorphic loci spanning Salmonella genome. The assay is a single-tube and single-step PCR reaction, where amplification and indexing take places simultaneously in the same reaction. The resolution of this assay is so high that it can distinguish most serotypes, but also can discriminate species of the same serotype. The assay can also detect up to four salmonella species of different serotypes from one sample. This assay is able to track-and-trace source of contamination. The data analysis software is included.
Technology
ChapterDx MLST Salmonella Serotyping NGS assay consists of the following steps:
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DNA extraction (not provided)
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Multiplex PCR
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Pooling and purification
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Next-generation sequencing
In the PCR reaction, both amplification and indexing/barcoding of each specimen occurs simultaneously. After PCR reaction, amplicons for different samples are pooled in one tube. The following procedures deal with only one tube regardless of how many samples. After the cleanup step, the library is sequenced on an Illumina sequencing platform. The entire workflow takes about 24 hours, of which about 3 hours is benchwork/incubation time and 17 hours of sequencing. It takes less than half hour to complete data analysis.
Features
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Analyze sequences for 46 polymorphic loci across Salmonella genome
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Distinguishes most Salmonella serotypes
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Discriminates species of the same serotype
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Detect up to four Salmonella of different serotypes from same sample
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Not only for serotyping samples, but also for tracking-and-tracing the source of contamination
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Single-tube and single-step PCR
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User-friendly workflow and easy to automate
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Cost-efficient, and suitable for low to high-throughput scales
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Data analysis software included
ChapterDx Salmonella Serotyping NGS Assay Workflow
