ChapterDX Campylobacter Target Enrichment
Reliable identification in just 24 hours
26 Pome Viruses/Viroids
ChapterDx PomePlex Target Enrichment NGS assay utilize a highly multiplexed approach to detect a panel of 26 pathogens affecting pome fruit. This assay replaces traditional methods by combining cDNA amplification with probe-based target enrichment in a specialized workflow
View the table listing all 26 Pome viruses/viroids detected using ChapterDX kit.
<14 Hours
The total workflow is designed for efficiency, requiring less than 14 hours to complete. This timeline includes 6 hours of benchwork and incubation time, approximately 7 hours of sequencing, and less than 30 minutes for automated data analysis.
Low amount of cDNA
This assay requires low amounts of input DNA and is suitable for small, medium and high-throughput sample scales.
Technology
The ChapterDx PomePlex Target Enrichment NGS Assay follows a streamlined workflow consisting of the following steps:
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DNA extraction (not provided)
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First PCR
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Pooling and purification
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Target enrichment
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Second PCR
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Pooling and purification
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Next-generation sequencing​
Chapter Diagnostics PomePlex Target Enrichment NGS assay is designed for high sensitivity using a dual-amplification strategy. cDNA samples are first amplified, then subjected to probe-based capture (Target Enrichment) using streptavidin beads. A second PCR step incorporates sequencing indexes. This method is suitable for small to high-throughput sample scales.
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This assay requires low amounts of input DNA and is suitable for small, medium and high-throughput sample scales. The assay procedure consists of the following steps: nucleic acid extraction (reagent not provided), reverse transcription, cDNA amplification, cleanup, target enrichment, amplification, cleanup, multiple sample pooling, and sequencing (reagent not provided). The turnaround time for the assay is less than 14 hours (6 hours laboratory procedures, 7 hours of DNA sequencing and 1 hour of data analysis).
26 Pome Viruses/Viroids
ChapterDx PomePlex Target Enrichment NGS Assay Workflow
