ChapterDX PomePlex-30
Reliable identification in just 12 hours
30 Pome Viruses/Viroids
The ChapterDX PomePlex-30 NGS kit utilizes a highly multiplexed PCR approach to detect a broad panel of pathogens affecting pome fruits such as apples, pears and quinces. This assay replaces traditional labor-intensive methods by targeting multiple targets per virus across the viral and viroid genomes in a single-tube reaction.
View the table listing all 30 Pome viruses/viroids detected using ChapterDX kit.
<12 Hours
The total workflow is designed for efficiency, requiring less than 12 hours to complete. This timeline includes 3-4 hours of benchwork and incubation time, approximately 7 hours of sequencing, and less than 30 minutes for automated data analysis.
Low amount of cDNA
This assay requires low amounts of input DNA and is suitable for small, medium and high-throughput sample scales.
Technology
The ChapterDX PomePlex-30 NGS kit utilizes a sophisticated, highly multiplexed PCR approach to revolutionize the detection of pathogens in Pome fruits, including apples, pears, and quinces. By replacing traditional, labor-intensive subtyping with a streamlined sequencing method optimized for Illumina platforms, this single-tube assay targets multiple loci across 30 different viral and viroid genomes simultaneously.
The workflow is engineered for maximum efficiency that follows a streamlined workflow consisting of the following steps: ​
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cDNA preparation (not provided)
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Amplification
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Pooling and purification
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Next-generation sequencing​
Designed for high sensitivity with low cDNA input, the assay is fully scalable for small, medium, and high-throughput laboratory environments. With a rapid total turnaround time of just 12 to 21 hours—including only 3–4 hours of hands-on lab procedures and 30 minutes of automated data analysis via proprietary software—the PomePlex-30 kit offers a professional, high-resolution solution for the comprehensive identification of pome variants. This technology provides a seamless, "sample-to-answer" experience that ensures precision and speed for modern molecular investigative approaches.
30 Pome Viruses/Viroids
ChapterDx PomePlex-30 NGS Assay Workflow
