26 Pome Viruses/Viroids
The ChapterDX PomePlex-26 NGS Kit utilizes a highly multiplexed PCR approach to detect a comprehensive panel of pome fruit viruses and viroids affecting apples, pears, and quinces. The assay targets 3–5 regions per virus for enhanced detection confidence and replaces traditional labor-intensive multiple single-tube PCR workflows with a streamlined, high-throughput solution.
View the table listing all 26 Pome viruses/viroids detected using ChapterDX kit.
<12 Hours
The total workflow is designed for efficiency, requiring less than 12 hours when using MiSeq i100. This timeline includes 3-4 hours of benchwork and incubation time, approximately 7 hours of sequencing, and less than 30 minutes for automated data analysis.
Low amount of cDNA
This assay requires low amounts of input cDNA and is suitable for small, medium and high-throughput sample scales.
Technology
The ChapterDX PomePlex-26 NGS Kit utilizes a highly multiplexed PCR approach for comprehensive detection of pome viruses and viroids in pome fruits, including apples, pears, and quinces.
Engineered for efficiency, the streamlined workflow includes:
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cDNA Preparation (not included)
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Amplification
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Purification
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Next-Generation Sequencing
The assay delivers a rapid total turnaround time of 12–21 hours, depending on the sequencing platform used, including only 3–4 hours of hands-on laboratory time and less than 30 minutes of automated data analysis using ChapterDX’s proprietary software.
The PomePlex-26 Kit provides a robust, high-throughput solution for comprehensive identification of pome fruit viruses and viroids.
26 Pome Viruses/Viroids
In addition to the 26 targeted pome fruit viruses and viroids, the ChapterDX PomePlex 26 panel also detects Phaseolus vulgaris alphaendornavirus 1 (PvEV-1) and Phaseolus vulgaris alphaendornavirus 2 (PvEV-2) as integrated positive controls, ensuring assay performance and reliable detection results.
ChapterDx PomePlex-26 NGS Assay Workflow
